| 产品介绍 | | |
| Mouse anti Human CD70 antibody, clone Bu69recognises human CD70, also known as CD27 ligand (CD27-L) or Tumor necrosis factor ligand superfamily member 7. CD70 is a 193 amino acid ~29 kDa single pass type II transmembrane glycoprotein belonging to the tumour necrosis factor (TNF) family (UniProt: P32970).CD70 is expressed on a small number of activated T-cells and the majority of activated B-cells (Bowmanet al.1994). Interaction of CD70 with its receptor, CD27, is thought to play an important role in lymphocyte activation, proliferation, survival and differentiation (Hintzenet al.1994).Mouse anti Human CD70 antibody, clone Bu69 has been used for the inhibition of neural stem cell induced apoptosis of allogeneic T cellsin vitro(Leeet al.2013). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09%Sodium Azide |
| Carrier Free | Yes |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/50 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) | STAR117A | F | 0.5mg |
| 文献 |
| 1.Garland Publishing. (1997) Leucocyte typing VI. White cell differentiation antigens.p522 - 42.Zambello, R.et al.(2000) Analysis of TNF-receptor and ligand superfamily molecules in patients with lymphoproliferative disease of granular lymphocytes.Blood. 96 (2): 647-54.3.Lee, E.M.et al.(2013) CD70-CD27 ligation between neural stem cells and CD4+ T cells induces Fas-FasL-mediated T-cell death.Stem Cell Res Ther. 4 (3): 56. |
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