Rat IgG2b kappa Isotype Control, Biotin
货号：13-4031-81,13-4031-82,13-4031-85
规格：50μg,100μg,500μg
价格：615,1132,2788
产品类型：大鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：大鼠
抗体亚型：IgG2b, kappa
克隆号：eB149/10H5
荧光染料：Biotin
抗体类型: | 单抗 | 同型对照： | IgG2b, kappa |
免疫原性： | | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow)；Assay-Dependent (IF);Assay-Dependent(ICC);Assay-Dependent(IHC) |
Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: Biotin Rat IgG2b Isotype Control has been reported for use in immunohistochemistry, immunocytochemistry and flow cytometric analysis.Applications Tested: Biotin Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. Use at the same concentration as the experimental antibody.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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参考文献： |
| 1.Nature medicine The pulmonary endothelial glycocalyx regulates neutrophil adhesion and lung injury during experimental sepsis. "13-4031 was used in Functional assays to elucidate the mechanisms by which glycocalyx loss occurs during sepsis, and how this allows for neutrophil adhesion within the pulmonary circulation." AuthorsSchmidt EP,Yang Y,Janssen WJ,Gandjeva A,Perez MJ,Barthel L,Zemans RL,Bowman JC,Koyanagi DE,Yunt ZX,Smith LP,Cheng SS,Overdier KH,Thompson KR,Geraci MW,Douglas IS,Pearse DB,Tuder RM |
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