IL-10 Human Instant ELISA Kit/人IL-10一步法ELISA试剂盒
货号：BMS215INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 0.66 pg/mL | 产品规格： | 128 tests |
检测范围： | 3.1-200 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | IL-10 |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 9.1% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 6.1% | 检测抗体偶联物： | Biotin |
| 同名基因： | CSIF, GVHDS, IL-10, IL10A, TGIF | 基因名称： | IL10 |
样品类型/体积： | 血清 50 µL 血浆 50µL 上清液 50µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Interleukin 10 (Hu IL-10) ELISA quantitates Hu IL-10 in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu IL-10.人白介素10（Hu IL-10）ELISA定量了人血清，血浆，细胞培养上清液或其他体液中的Hu IL-10。 该检测试剂盒只识别天然和重组的Hu IL-10。Principle of the method 方法原理The Human IL-10 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-10夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.白介素10是一种多效细胞因子，在调节淋巴和髓样细胞功能中起着重要作用。在人类中，IL-10由活化的CD8（+）外周血T细胞，T辅助CD4（+）T细胞克隆产生。由于IL-10具有阻断细胞因子合成的能力以及巨噬细胞的几种辅助细胞功能，因此该细胞因子是巨噬细胞，T细胞和NK细胞的效应子功能的有效抑制剂。另外，IL-10参与调节B细胞，肥大细胞和胸腺细胞的增殖和分化。已经通过克隆编码细胞因子的cDNA来确定人IL-10的一级结构。相应的蛋白质具有160个氨基酸，预测分子量为18.5 kDa。根据其主要结构，IL-10是细胞因子的四个a-螺旋束家族的成员。在溶液中，人IL-10是表观分子量为39kDa的同型二聚体。尽管它包含一个N连接的糖基化位点，但它缺乏可检测的碳水化合物。因此，在大肠杆菌中表达的重组蛋白保留了所有已知的生物学活性。人IL-10基因位于1号染色体上，在基因组中以单拷贝形式存在。人IL-10与鼠类IL-10表现出很强的DNA和氨基酸序列同源性，并在爱泼斯坦-巴尔病毒基因组BCRF1中具有开放阅读框，BCRF1具有许多细胞因子的生物学活性，因此可能在其中发挥作用。主机与病毒的交互。 IL-10的免疫抑制特性表明IL-10在抑制器官移植后移植物排斥反应方面的临床应用可能。 IL-10还可以发挥强大的抗炎活性。仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
IL-10 Human Instant ELISA™ KitRepresentative standard curve for Human IL-10 Instant ELISA |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-17196.htm
QQ:1749072012