T4 RNA Ligase 2, Deletion Mutant T4 RNA连接酶2，缺失突变（停产））
货号：LR2D11310K,LR2D1132K
规格：10000 U 200 U/µL,2000 U 200 U/µL
价格：3958,1260
产品类型：Modifying Enzymes连接酶
品牌：Lucigen
| T4 RNA Ligase 2,Deletion Mutant, also known as T4Rnl2(1-249), is used to ligate singlestranded adenylated DNA or RNA oligonucleotides to small RNAs for cloning or nextgeneration RNA sequencing. The preadenylated 5′ ends of DNA or RNA are ligated to the 3′ ends of RNA. Unlike the full-length enzyme, T4Rnl2(1-249) is unable to adenylate the 5′ end of the substrate in the presence of ATP. However, it can use a preactivated donor(App-DNA or App-RNA) and join it to the 3′ end of an acceptor; thus, performing the ligation reaction in the absence of ATP prevents circularization and other undesirable bimolecular reactions. The enzyme is active in a pH range of 6.0 to 8.0.T4 RNA 连接酶2，缺失突变,T4Rn 12(1-249)，将单链腺苷酸化DNA或RNA（App-RNA）寡核苷酸连接至小RNA。在不存在ATP的情况下，将DNA或RNA的预腺苷酸化的5，末端连接到RNA的3，末端，这样防止了环化和其它不需要的双分子反应。 |
| 优点： |
| ▪ Ligate single-stranded adenylated DNA or RNA oligos to RNA targets▪ Use this enzyme to prepare cDNA libraries for small-RNA transcriptome analysis such as RNA-Seq▪ Perform linker ligation for miRNA cloning applications |
| 组成成分： |
| ▪ T4 RNA Ligase 2 Deletion Mutant (200 U/µl) :储存在-20℃▪ 10X T4 RNA Ligase 2 Deletion Mutant Reaction Buffer:储存在-20℃▪ T4 RNA Ligase 2 Deletion Mutant (200 U/µl) :储存在-20℃▪ 10X T4 RNA Ligase 2 Deletion Mutant Reaction Buffer :储存在-20℃ |
技术参数 产品优点 - Ligate single-stranded adenylated DNA or RNA oligos to RNA targets
- Use this enzyme to prepare cDNA libraries for small-RNA transcriptome analysis such as RNA-Seq
- Perform linker ligation for miRNA cloning applications
产品应用 - Preparing cDNA libraries for small-RNA transcriptome analysis.
- Providing optimal linker ligation for miRNA cloning.
- 制备cDNA文库用于小RNA转录组分析，例如RNA-Seq
- 用于miRNA克隆的最佳接头连接
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