CD25 Monoclonal Antibody (BC96), PE, eBioscience™

货号：12-0259-41,12-0259-42

规格：25 tests,100 tests

价格：1046,1072

产品类型：流式抗体

品牌：eBioscience

抗原：CD25

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG

克隆号：BC96

荧光染料：PE

抗体类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	5uL（0.125 µg）/test(Flow）

Description: The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.Applications Reported: The BC96 antibody has been reported for use in flow cytometric analysis.Applications Tested: The BC96 antibody is offered in 2 formats:- µg size: It can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.- test size: has been pre-titrated and tested and can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD25 Antibody (12-0259-41) in FlowStaining of PHA-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) and Anti-Human CD25 PE (filled histogram).Total viable cells were used for analysis.

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参考文献：

1.PloS oneImpact of Pre-Transplant Anti-T Cell Globulin (ATG) on Immune Recovery after Myeloablative Allogeneic Peripheral Blood Stem Cell Transplantation."12-0259 was used in Flow cytometry/Cell sorting to assess the impact of anti-T cell globulin on the recovery of a large variety of lymphoid cell subsets as well as on thymic function after myeloablative peripheral blood stem cell transplantation."AuthorsServais S,Menten-Dedoyart C,Beguin Y,Seidel L,Gothot A,Daulne C,Willems E,Delens L,Humblet-Baron S,Hannon M,Baron F2.Scientific reportsCRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients."12-0259 was used in Flow cytometry/Cell sorting to explore the effects of genetic disruption of the gene PD-1 on T cell viability, IFN-γ production and cytotoxicity."AuthorsSu S,Hu B,Shao J,Shen B,Du J,Du Y,Zhou J,Yu L,Zhang L,Chen F,Sha H,Cheng L,Meng F,Zou Z,Huang X,Liu B3.Science signalingChemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells."12-0259 was used in Flow cytometry/Cell sorting to explore the mode of action of Dimethyl fumarate as a therapy for autoimmune diseases."AuthorsBlewett MM,Xie J,Zaro BW,Backus KM,Altman A,Teijaro JR,Cravatt BF4.PloS oneAge-Associated Differences in MiRNA Signatures Are Restricted to CD45RO Negative T Cells and Are Associated with Changes in the Cellular Composition, Activation and Cellular Ageing."12-0259 was used in Flow cytometry/Cell sorting to show that ageing related changes in miRNA expression are dominant in the CD45RO- T-cell compartment."AuthorsTeteloshvili N,Kluiver J,van der Geest KS,van der Lei RJ,Jellema P,Pawelec G,Brouwer E,Kroesen BJ,Boots AM,van den Berg A5.PloS oneNeuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs."12-0259 was used in Flow cytometry/Cell sorting to characterise Nrp1-expressing T cells in human secondary lymphoid organs."AuthorsRenand A,Milpied P,Rossignol J,Bruneau J,Lemonnier F,Dussiot M,Coulon S,Hermine O

技术参数

产品应用 Flow

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