Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 700/羊抗兔IgG (H+L)交叉吸附，Alexa Fluor 700

货号：A-21038

规格：500μl

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 700

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL（Flow）；1-10 μg/mL (ICC)；1-10 μg/mL (IF)； 0.04-0.2 μg/mL (WB)

This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21038) in WBWestern blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1) and U-87 MG (Lane 2). The blots were probed with ABfinity™ Anti-JUNB Recombinant Rabbit Monoclonal Antibody (Product # 701702, 2 µg/mL) and detected using Goat anti-Rabbit IgG (H+L) cross adsorbed Secondary Antibody, Alexa Fluor 700 (Product # A-21038) at concentrations 0.04 µg/mL (Fig. 1), 0.1 µg/mL (Fig. 2) and 0.2 µg/mL (Fig. 3). A 42 kDa band corresponding to JUNB was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

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参考文献：

1. American journal of physiology. Regulatory, integrative and comparative physiologyNitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant rats."A21038 was used in western blot to study the effect of nitric oxide on the blood pressure of pregnant rats"AuthorsSpradley FT,Sasser JM,Musall JB,Sullivan JC,Granger JP2. The EMBO journalFunctioning of the dimeric GABA(B) receptor extracellular domain revealed by glycan wedge scanning.AuthorsRondard P,Huang S,Monnier C,Tu H,Blanchard B,Oueslati N,Malhaire F,Li Y,Trinquet E,Labesse G,Pin JP,Liu J

技术参数

产品应用 ICC;IF;WB;Flow

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