Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680/羊抗鼠 IgG (H+L) 高交叉吸附 荧光二抗, Alexa Fluor 680

货号：A-21058

规格：500μl

价格：3719

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 680

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
浓度：	2mg/mL	用法：	1-10 μg/mL (ICC)；1-10 μg/mL (IF)； 1:5000-1:10000 μg/mL (WB)

产品详细信息This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21058) in WBGoat anti-Mouse IgG (H+L) Alexa Fluor 680 Secondary Antibody in WB Western blot analysis of total Cadherin and N-Cadherin was performed by loading 2 µL SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925), 50 µg of MDCK cell lysates and 10 µg mouse heart lysate per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product # 71-7100) and N-Cadherin was detected using a mouse antibody (Product # 33-3900), both at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with goat anti-rabbit IgG-Alexa Fluor 790 secondary antibody (Product # A11369) and goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product # A-21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center. Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21058) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1) and HeLa (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105, 2 µg/mL) and detected using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 680 (Product # A-21058) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:20,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences). Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-21058) in ICC Western blot analysis of total Cadherin and P-Cadherin was performed by loading and 2 µL SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925), 50 µg of MDCK cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product # 71-7100) and P-Cadherin was detected using a mouse antibody (Product # 32-4000), both at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with a goat anti-rabbit IgG-Alexa fluor 790 secondary antibody (Product # A11369) and a goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product # A-21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Images is generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.

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参考文献：

1. PloS oneDistinct repertoires of microRNAs present in mouse astrocytes compared to astrocyte-secreted exosomes.2. FASEB journal : official publication of the Federation of American Societies for Experimental BiologyRegulated intramembrane proteolysis of the AXL receptor kinase generates an intracellular domain that localizes in the nucleus of cancer cells.3. OncotargetAndrogen receptor transcriptionally regulates semaphorin 3C in a GATA2-dependent manner.4. Proceedings of the National Academy of Sciences of the United States of AmericaLoss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration.5. JCI insightEpigenetic regulation of macrophage polarization and inflammation by DNA methylation in obesity.

技术参数

产品应用 ICC;IF;WB

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