Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 647/羊抗鸡 IgY (H+L) 荧光二抗, Alexa Fluor 647

货号：A-21449

规格：500μl

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

物种：其它

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 647

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10 μg/mL (ICC)；1-10 μg/mL (IF)； 0.04-0.2 μg/mL (IHC)

These goat anti-chicken IgY (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

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参考文献：

1. NatureCell diversity and network dynamics in photosensitive human brain organoids."A21449 was used in immunocytochemistry to compare the gene expression in individual cells isolated from human brain organoids"AuthorsQuadrato G,Nguyen T,Macosko EZ,Sherwood JL,Min Yang S,Berger DR,Maria N,Scholvin J,Goldman M,Kinney JP,Boyden ES,Lichtman JW,Williams ZM,McCarroll SA,Arlotta P2．HippocampusN-terminal SAP97 isoforms differentially regulate synaptic structure and postsynaptic surface pools of AMPA receptors."A21449 was used in immunocytochemistry to investigate diverging presynaptic and postsynaptic roles of SAP97 N-terminal isoforms in synapse maturation and plasticity"AuthorsGoodman L,Baddeley D,Ambroziak W,Waites CL,Garner CC,Soeller C,Montgomery JM3. Nature communicationsR function and expression with rapid antidepressant properties."A-21449 was used in immunocytochemistry to investigate how acute ethanol exposure results in lasting antidepressant and anxiolytic behaviors"AuthorsWolfe SA,Workman ER,Heaney CF,Niere F,Namjoshi S,Cacheaux LP,Farris SP,Drew MR,Zemelman BV,Harris RA,Raab-Graham KF4. Stem cell researchHiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells."A21449 was used in immunocytochemistry to examine c-kit expression and localization in the murine heart"AuthorsShi H,Drummond CA,Fan X,Haller ST,Liu J,Malhotra D,Tian J5. The Journal of general physiologyA novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes."A21449 was used in western blot to develop methods to study vesicle-associated proteins and exocytosis in stellate astrocytes"AuthorsWolfes AC,Ahmed S,Awasthi A,Stahlberg MA,Rajput A,Magruder DS,Bonn S,Dean C

技术参数

产品应用 ICC;IF;IHC

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