CD3e Monoclonal Antibody (145-2C11)

货号：14-0031-82,14-0031-85,14-0031-86

规格：100μg,500μg,1mg

价格：807,2066,3767

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：145-2C11

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG
免疫原性：		用法：	0.5 μg/test(Flow)；Assay-Dependent (FN)；Assay-Dependent（WB）；Assay-Dependent (IP)

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in cell activation and cell depletion. (Please use Functional Grade purified 145-2C11, cat. 16-0031, in functional assays.).Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3e Antibody (14-0031-82) in FlowStaining of C57Bl/6 splenocytes with Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or Anti-Mouse CD3e Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

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参考文献：

1. JCI insightPeroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease."14-0031 was used in Functional assays to demonstrate that β-oxidation links metabolism and immune responsiveness."AuthorsMoreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S2. PloS onePurinergic Signaling as a Regulator of Th17 Cell Plasticity."14-0031 was used in Functional assays to suggest that CD39 expression by Th17 cells allows depletion of ATP facilitates IL-10 production and survival during intestinal inflammation resolution."AuthorsFernández D,Flores-Santibáñez F,Neira J,Osorio-Barrios F,Tejón G,Nuñez S,Hidalgo Y,Fuenzalida MJ,Meza D,Ureta G,Lladser A,Pacheco R,Acuña-Castillo C,Guixé V,Quintana FJ,Bono MR,Rosemblatt M,Sauma D3. eLifePKCθ links proximal T cell and Notch signaling through localized regulation of the actin cytoskeleton."14-0031 was used in Functional assays to investigate the role of protein kinase PKCθ in linking proximal T cell and notch signaling by a spatiotemporally constrained mechanism."AuthorsBritton GJ,Ambler R,Clark DJ,Hill EV,Tunbridge HM,McNally KE,Burton BR,Butterweck P,Sabatos-Peyton C,Hampton-O'Neil LA,Verkade P,Wülfing ChC,Wraith DC4. Science advancesCitrullination-acetylation interplay guides E2F-1 activity during the inflammatory response."14-0031 was used in Functional assays to investigate the mechanism of action of PAD4."AuthorsGhari F,Quirke AM,Munro S,Kawalkowska J,Picaud S,McGouran J,Subramanian V,Muth A,Williams R,Kessler B,Thompson PR,Fillipakopoulos P,Knapp S,Venables PJ,La Thangue NB5. Journal of neuroinflammationRGS10 deficiency ameliorates the severity of disease in experimental autoimmune encephalomyelitis."14-0031 was used in Functional assays to define potential roles for RGS10 in regulating autoimmune responses, showing that deficiency ameliorates the severity of disease in EAE."AuthorsLee JK,Kannarkat GT,Chung J,Joon Lee H,Graham KL,Tansey MG

技术参数

产品应用 FN;WB;IP;Flow

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