Rat IgG1 kappa Isotype Control (eBRG1), PE

货号：12-4301-81,12-4301-82,12-4301-83

规格：50μg,100μg,200μg

价格：987,1632,2448

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：PE

抗体类型:	单抗	同型对照：	IgG1, kappa
免疫原性：		用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis.Applications Tested: The PE rat IgG1 isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at the same concntration as the experimental antibody.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1. Oncology lettersMicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cells."12-4301 was used in Flow cytometry/Cell sorting to observe the effect on the tumour-targeting capacity of cytokine-induced killer cells, following miR-374b regulation of PD-1 expression."AuthorsHuang F,Wang B,Zeng J,Sang S,Lei J,Lu Y2. Nature communicationsαv integrins on mesenchymal cells regulate skeletal and cardiac muscle fibrosis."12-4301 was used in Flow cytometry/Cell sorting to show that PDGFRβ+cells contribute to skeletal muscle and cardiac fibrosis via a mechanism that depends on αv integrins."AuthorsMurray IR,Gonzalez ZN,Baily J,Dobie R,Wallace RJ,Mackinnon AC,Smith JR,Greenhalgh SN,Thompson AI,Conroy KP,Griggs DW,Ruminski PG,Gray GA,Singh M,Campbell MA,Kendall TJ,Dai J,Li Y,Iredale JP,Simpson H,Huard J,Péault B,Henderson NC3. Nature communicationsPericyte-expressed Tie2 controls angiogenesis and vessel maturation."12-4301 was used in Flow cytometry/Cell sorting to expand and revise the current concepts on vascular Angiopoietin/Tie signalling and propose a bidirectional, reciprocal EC-pericyte model of Tie2 signalling."AuthorsTeichert M,Milde L,Holm A,Stanicek L,Gengenbacher N,Savant S,Ruckdeschel T,Hasanov Z,Srivastava K,Hu J,Hertel S,Bartol A,Schlereth K,Augustin HG4. Experimental and therapeutic medicineChitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats."12-4301 was used in Flow cytometry/Cell sorting to evaluate the suitability of chitosan hydrogel, an injectable scaffold, to deliver mesenchymal stem cells into infarcted myocardium."AuthorsXu B,Li Y,Deng B,Liu X,Wang L,Zhu QL5. Scientific reportsT cells Immune Responses."12-4301 was used in Flow cytometry/Cell sorting to indicate that respiratory syncytial virus infection can increase C5a and C5aR expression in the pathogenesis of infected, asthmatic mice."AuthorsHu X,Li X,Hu C,Qin L,He R,Luo L,Tang W,Feng J

技术参数

产品应用 Flow

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