CD25 Monoclonal Antibody (PC61.5), PE
货号：12-0251-81,12-0251-82,12-0251-83
规格：50μg,100μg,200μg
价格：1029,2019,2840
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：PC61.5
荧光染料：PE
抗体类型: | 小鼠流式抗体 | 同型对照： | Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience |
浓度： | 0.2mg/mL | 用法： | 0.125 μg/test(Flow) |
Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes and Con A-stimulated splenocyte culture. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
数据 |
| CD25 Antibody (12-0251-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.06 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (left) or 0.06 µg of Anti-Mouse CD25 PE (right). Total viable cells were used for analysis. |
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参考文献： |
| 1. Frontiers in neurologyIL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury."12-0251 was used in Flow cytometry/Cell sorting to suggest that IL-2C treatment can alleviate inflammation and maintain blood vessel stability after traumatic brain injury."AuthorsGao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J2. OncoimmunologyInterleukin-21 combined with PD-1 or CTLA-4 blockade enhances antitumor immunity in mouse tumor models."12-0251 was used in Flow cytometry/Cell sorting to investigate the efficacy of immune-modulatory interleukin-21 combined with checkpoint blockade in several syngeneic mouse tumor models."AuthorsLewis KE,Selby MJ,Masters G,Valle J,Dito G,Curtis WR,Garcia R,Mink KA,Waggie KS,Holdren MS,Grosso JF,Korman AJ,Jure-Kunkel M,Dillon SR3. Nature communicationsItk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4."12-0251 was used in Magnetic cell separation to investigate the role of IL-9 in allergic asthma and autoimmunity, showing that Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4."AuthorsGomez-Rodriguez J,Meylan F,Handon R,Hayes ET,Anderson SM,Kirby MR,Siegel RM,Schwartzberg PL4. International immunologyAn MHC-linked locus modulates thymic differentiation of CD4+CD25+Foxp3+ regulatory T lymphocytes."12-0251 was used in Flow cytometry/Cell sorting to investigate factors that affect the levels of Tregs in the thymus."AuthorsTellier J,van Meerwijk JP,Romagnoli P5. Molecular and cellular biologyIncreased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection."12-0251 was used in Western Blotting to determine the effects of increased p300 expression on GR-TCR crosstalk between thymocytes."AuthorsYu CT,Feng MH,Shih HM,Lai MZ |
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