Goat anti-Rabbit IgG (H+L) Cross-Adsorbed ReadyProbes™ Secondary Antibody, Alexa Fluor 594

货号：R37117

规格：2 x 2.5 mL

价格：1769

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 594

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobin	用法：	Assay-Dependent (ICC);Assay-Dependent (IF);Assay-Dependent（Flow）

No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.Background/Target InformationAnti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (R37117) in IFCAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (Product # R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent (Product # R37117) for 30 min and washed three times in dPBS. Cells were labeled with NucBlue® Live (Product # R37605) and ActinGreen™ 488 ReadyProbes® reagent (Product # R37110) according to protocol. Coverslips were then mounted using ProLong® Gold antifade reagent (Product # P36930). Individual images were acquired on the EVOS® FL Auto imaging system with a 10X objective (Product # AMEP4681) and stitched to a panorama using the tile/stitch function.

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参考文献：

1. F1000ResearchReduced neuronal size and mTOR pathway activity in the Mecp2 A140V Rett syndrome mouse model."R37117 was used in immunocytochemistry to create and characterize a mouse model that expresses the recurrent human MECP2A140V mutation that is linked to an X-linked mental retardation/Rett syndrome phenotype"AuthorsRangasamy S,Olfers S,Gerald B,Hilbert A,Svejda S,Narayanan V2. F1000ResearchReduced neuronal size and mTOR pathway activity in the Mecp2 A140V Rett syndrome mouse model."R37117 was used in immunocytochemistry to create and characterize a mouse model that expresses the recurrent human MECP2A140V mutation that is linked to an X-linked mental retardation/Rett syndrome phenotype"AuthorsRangasamy S,Olfers S,Gerald B,Hilbert A,Svejda S,Narayanan V3. Nature structural and molecular biologyE4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis."R37117 was used in immunocytochemistry to find a role for UFD-2 in DNA-damage-induced apoptosis"AuthorsAckermann L,Schell M,Pokrzywa W,Kevei É,Gartner A,Schumacher B,Hoppe T4. Nature methodsAn E3-ligase-based method for ablating inhibitory synapses."R37117 was used in immunocytochemistry to develop a simple, reversible method for modulating inhibitory synaptic input onto genetically determined cells"AuthorsGross GG,Straub C,Perez-Sanchez J,Dempsey WP,Junge JA,Roberts RW,Trinh le A,Fraser SE,De Koninck Y,De Koninck P,Sabatini BL,Arnold DB5. Journal of cell scienceControl of apico-basal epithelial polarity by the microtubule minus-end-binding protein CAMSAP3 and spectraplakin ACF7."R37117 was used in immunocytochemistry to show that ACF7 governs apico-basal polarity in mammalian intestinal cells"AuthorsNoordstra I,Liu Q,Nijenhuis W,Hua S,Jiang K,Baars M,Remmelzwaal S,Martin M,Kapitein LC,Akhmanova A

技术参数

产品应用 ICC;IF;Flow

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