PARP (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared / PARP（切割）多物种细胞内ELISA试剂盒，近红外

货号：62224

规格：96 tests

价格：7066

产品类型：ELISA检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

The Cleaved PARP Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells.Principle of the methodThis kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively.Rigorous validationEach manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Target informationPoly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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