F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555/F(ab')2-山羊抗小鼠IgG (H+L)交叉吸附二抗，Alexa Fluor 555

货号：A-21425

规格：500 µg

价格：2787

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 555

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	1-10 μg/mL（Flow）；2 μg/mL (ICC)；2 μg/mL (IF)

点击查看所有Alexa Fluor 荧光二抗

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21425) in IFImmunofluorescence analysis of F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 conjugate was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 555 (Product # A-21425) was used at a concentration of 2 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21425) in IFHemin induced lipid peroxidation and nrf-2 nuclear translocation in RAW macrophage cells. Detection of lipid peroxidation was done by click reaction with AlexaFluor® 488 azide using Click-iT® Lipid Peroxidation Imaging Kit - Alexa Fluor® 488 (Product # C10446) and nrf-2 by using an antibody against nrf-2 and AlexaFluor® 555 secondary antibody. Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-21425) in IFHemin induced lipid peroxidation in bovine pulmonary artery endothelial cells. Detection of lipid peroxidation was by click reaction with AlexaFluor 488® azide and hydroxynonenal (HNE) was detected by using an antibody against HNE and AlexaFluor® 555 secondary antibody.

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参考文献：

1. Journal of cell scienceError-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin.2. OncotargetLY3009120, a panRAF inhibitor, has significant anti-tumor activity in BRAF and KRAS mutant preclinical models of colorectal cancer.3. Cell transplantationDirected Differentiation of Oligodendrocyte Progenitor Cells From Mouse Induced Pluripotent Stem Cells.4. Molecular biology of the cellSpecific functional pathologies of Cx43 mutations associated with oculodentodigital dysplasia.5. Biomedical optics expressSimple buffers for 3D STORM microscopy.

技术参数

产品应用 ICC;IF;Flow

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