Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555/驴抗兔 (H+L)高交叉吸附荧光二抗 , Alexa Fluor 555

货号：A-31572

规格：500μl

价格：3589

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：驴

抗体亚型：IgG

荧光染料：Alexa Fluor 555

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	Assay-Dependent（Flow）；4 μg/mL (ICC)；4 μg/mL (IF)； 1-10 μg/mL (IHC)

These donkey anti-rabbit IgG (H+L)whole secondary antibodies have been affinity-purified and show minimum cross-reactivity. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 555 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

数据

Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A-31572) in IFImmunofluorescence analysis of Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 555 conjugate was performed using HeLa cells stained with alpha Tubulin Rabbit Polyclonal Antibody (Product # PA516891) The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with 2 µg/mL primary antibody for 3 hours at room temperature. Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor® 555 conjugate (Product # A-31572) was used at a concentration of 4 µg/mL in phosphate buffered saline containing 0.2% BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: red). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379), 1:300) (Panel c: green). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

推荐产品

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Frontiers in neuroanatomyAdeno-Associated Viral Vectors Serotype 8 for Cell-Specific Delivery of Therapeutic Genes in the Central Nervous System."A31572 was used in immunohistochemistry - frozen section to generate AAV vectors designed for specific expression in cells of the CNS using minimal promoters to drive gene expression"AuthorsPignataro D,Sucunza D,Vanrell L,Lopez-Franco E,Dopeso-Reyes IG,Vales A,Hommel M,Rico AJ,Lanciego JL,Gonzalez-Aseguinolaza G2. Journal of neuroinflammationIntracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model."A-31572 was used in immunohistochemistry - frozen section to develop an in vivo approach using intracerebral grafting of mesenchymal stem cells to secrete interleukin 13"AuthorsLe Blon D,Guglielmetti C,Hoornaert C,Quarta A,Daans J,Dooley D,Lemmens E,Praet J,De Vocht N,Reekmans K,Santermans E,Hens N,Goossens H,Verhoye M,Van der Linden A,Berneman Z,Hendrix S,Ponsaerts P3. Neural developmentThe methyl binding domain 3/nucleosome remodelling and deacetylase complex regulates neural cell fate determination and terminal differentiation in the cerebral cortex."A-31572 was used in immunohistochemistry - frozen section to investigate the role of MBD3/NuRD in neurogenesis."AuthorsKnock E,Pereira J,Lombard PD,Dimond A,Leaford D,Livesey FJ,Hendrich B4. BMC neuroscienceBoundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents."A-31572 was used in immunohistochemistry - frozen section to study the fate of mouse boundary cap neural crest stem cells implanted in the dorsal root transitional zone"AuthorsTrolle C,Konig N,Abrahamsson N,Vasylovska S,Kozlova EN5. The Journal of neuroscience : the official journal of the Society for NeuroscienceFoxg1 has an essential role in postnatal development of the dentate gyrus."A-31572 was used in immunohistochemistry - frozen section to determine the function of Foxg1 in postnatal dentate gyrus neurogenesis."AuthorsTian C,Gong Y,Yang Y,Shen W,Wang K,Liu J,Xu B,Zhao J,Zhao C

技术参数

产品应用 ICC;IF;IHC;Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17395.htm