Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 790
货号：A27041
规格：1mg
价格：3319
产品类型：荧光二抗
品牌：Thermo Fisher
物种：兔
宿主：山羊
抗体亚型：IgG
荧光染料：Alexa Fluor 555
抗体类型: | 荧光二抗 | 同型对照： | IgG |
免疫原性： | Recombinant full-length protein | 用法： | 0.5-2 µg/mL (WB) |
The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed.Background/Target InformationSuperclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
数据 |
| Rabbit IgG (H+L) Secondary Antibody (A27041) in WBEndogenous beta-actin (A) and endogenous MEK2 (B) was detected using Rabbit anti-beta-actin and Rabbit anti-MEK2 (Product # 700829) primary antibody, and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 790 Conjugate (Product # A27041) (0.4 µg/mL 1:2500 dilution). No primary control to assess background (C) and loading control (LC) mouse anti-Tubulin (Product # 32-2500) is shown. Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa human cervical carcinoma cells (lane 1-5) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. |
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参考文献： |
| 1. Molecular medicine reportsNatural compound oblongifolin C inhibits autophagic flux, and induces apoptosis and mitochondrial dysfunction in human cholangiocarcinoma QBC939 cells."A27041 was used in western blot to evaluate the effects of Oblongifolin C on autophagy and mitochondrial dysfunction in cholangiocarcinoma cells"AuthorsZhang A,He W,Shi H,Huang X,Ji G2. Molecular medicine reportsAutophagy is involved in aldosterone‑induced mesangial cell proliferation."A27041 was used in western blot to test if autophagy contributes to aldosterone-induced mesangial cell proliferation"AuthorsYang M,Wang B,Miao L,Xu X,He X |
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