CD69 Monoclonal Antibody (H1.2F3), eBioscience™

货号：14-0691-82

规格：100 µg

价格：1260

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：Purified

类型:	一抗	同型对照：	IgG
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: The H1.2F3antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. H1.2F3 has also been reported in in vitro cell activation. (Please use Functional Grade purified H1.2F3, cat. 16-0691, in functional assays.).Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。

数据

CD69 Antibody (14-0691-82) in FlowSurface staining of ConA-stimulated splenocytes with Anti-Mouse CD69 PE (Product # 12-0691-82). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

1. The Journal of experimental medicineType I IFN enhances follicular B cell contribution to the T cell-independent antibody response."14-0691 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which toll-like receptor ligands influence T cell-independent type 2 antibody responses."AuthorsSwanson CL,Wilson TJ,Strauch P,Colonna M,Pelanda R,Torres RMYear2010SpeciesMouse2. Journal of immunology (Baltimore, Md. : 1950)Depleting syndecan-4+ T lymphocytes using toxin-bearing dendritic cell-associated heparan sulfate proteoglycan-dependent integrin ligand: a new opportunity for treating activated T cell-driven disease."14-0691 was used in Flow cytometry/Cell sorting to investigate whether the DC-HIL/SD-4 pathway could be a therapeutic target for conditions mediated by selectively activated T cells."AuthorsAkiyoshi H,Chung JS,Tomihari M,Cruz PD,Ariizumi KYear2010SpeciesMouse3. Journal of immunology (Baltimore, Md. : 1950)ATP-binding cassette transporter G1 negatively regulates thymocyte and peripheral lymphocyte proliferation."14-0691 was used in Flow cytometry/Cell sorting to demonstrate that ATP-binding cassette transporter G1 regulates cholesterol homeostasis in thymocytes and peripheral CD4 T cells."AuthorsArmstrong AJ,Gebre AK,Parks JS,Hedrick CCYear2010SpeciesMouse4. CellPI3 kinase signals BCR-dependent mature B cell survival."14-0691 was used in Flow cytometry/Cell sorting to identify the molecular nature of the essential B cell survival signal delivered to BCRs."AuthorsSrinivasan L,Sasaki Y,Calado DP,Zhang B,Paik JH,DePinho RA,Kutok JL,Kearney JF,Otipoby KL,Rajewsky KYear2009SpeciesMouse5. PLoS pathogensCoxsackievirus B3 inhibits antigen presentation in vivo, exerting a profound and selective effect on the MHC class I pathway."14-0691 was used in Flow cytometry/Cell sorting to evaluate in vitro and in vivo antigen presentation by coxsackievirus B3, showing that it inhibits antigen presentation in vivo."AuthorsKemball CC,Harkins S,Whitmire JK,Flynn CT,Feuer R,Whitton JL

技术参数

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