Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 546/驴抗羊 IgG (H+L)交叉吸附荧光二抗 ，Alexa Fluor 546

货号：A-11056

规格：500 µL

价格：3589

产品类型：荧光二抗

品牌：Thermo Fisher

物种：山羊

宿主：驴

抗体亚型：IgG

荧光染料：Alexa Fluor 546

点击查看所有Alexa Fluor荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobins Heavy and Light chains	用法：	1-10µg/mL(ICC);1-10µg/mL（IF); 1-10µg/mL(IHC);1-10µg/mL（Flow)

To minimize cross-reactivity, these donkey anti-goat IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against rabbit, rat, mouse, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Journal of tissue engineering and regenerative medicineCorneal keratocyte transition to mesenchymal stem cell phenotype and reversal using serum-free medium supplemented with fibroblast growth factor-2, transforming growth factor-β3 and retinoic acid."A11056 was used in immunocytochemistry to explore factors that induce the transition of keratocytes to corneal mesenchymal stem cells and vice versa"AuthorsSidney LE,Hopkinson A2.NatureCell diversity and network dynamics in photosensitive human brain organoids."A11056 was used in immunocytochemistry to compare the gene expression in individual cells isolated from human brain organoids"AuthorsQuadrato G,Nguyen T,Macosko EZ,Sherwood JL,Min Yang S,Berger DR,Maria N,Scholvin J,Goldman M,Kinney JP,Boyden ES,Lichtman JW,Williams ZM,McCarroll SA,Arlotta P3.AutophagyFYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules."A11056 was used in immunohistochemistry to investigate how FYCO1 contributes to the integrity of the chromatoid body"AuthorsDa Ros M,Lehtiniemi T,Olotu O,Fischer D,Zhang FP,Vihinen H,Jokitalo E,Sironen A,Toppari J,Kotaja N4.PloS oneMHC-I and PirB Upregulation in the Central and Peripheral Nervous System following Sciatic Nerve Injury."A11056 was used in immunohistochemistry to measure expression of major histocompatibility complex class one and paired immunoglobulin-like receptor B in the central and peripheral nervous system after sciatic nerve injury in mice"AuthorsBombeiro AL,Thomé R,Oliveira Nunes SL,Monteiro Moreira B,Verinaud L,Oliveira AL5.Journal of neuropathology and experimental neurologyNeuronal Injury, Gliosis, and Glial Proliferation in Two Models of Temporal Lobe Epilepsy."A11056 was used in immunohistochemistry to characterize and measure acute neuronal injury and inflammatory features in two mouse models of temporal lobe epilepsy"AuthorsLoewen JL,Barker-Haliski ML,Dahle EJ,White HS,Wilcox KS

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