CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), PE

货号：12-2099-42

规格：100 Tests

价格：2987

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eB-h209

荧光染料：PE

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
免疫原性：		用法：	5uL（0.06µg）/test(Flow）

Description: The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.Applications Reported: The eB-h209 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThis gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

数据

CD209 (DC-SIGN) Antibody (12-2099-42) in FlowStaining of human monocyte-derived immature dendritic cells with Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or Anti-Human CD209 (DC-SIGN) PE (purple histogram). Cells in the large forward scatter population were used for analysis.

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参考文献：

1. Mediators of inflammationThe Correlation of CD206, CD209, and Disease Severity in Behçet's Disease with Arthritis."12-2099 was used in Flow cytometry/Cell sorting to analyse the expression of CD206, CD209, and Dectin-1 in active and inactive Behcet's disease patients with arthritis."AuthorsChoi B,Suh CH,Kim HA,Sayeed HM,Sohn S2. Frontiers in immunologyLeishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System."12-2099 was used in Flow cytometry/Cell sorting to investigate the similarities and differences between the ways in which leishmania infantum and leishmania braziliensis evade the innate immune system.."AuthorsFalcão Sde A,Jaramillo TM,Ferreira LG,Bernardes DM,Santana JM,Favali CB3. PLoS pathogensPorphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. PLoS neglected tropical diseasesKinetic analysis of ex vivo human blood infection by Leishmania."12-2099 was used in Flow cytometry/Cell sorting to show that promastigote invasion of human leukocytes is extremely rapid, efficient and is key for subverting host innate immune defences."AuthorsMoreno I,Domínguez M,Cabañes D,Aizpurua C,Toraño A

技术参数

产品应用 Flow

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