Attune® Focusing Fluid is an optimized buffer solution that is used in the Attune® Acoustic Focusing Cytometer for focusing and transportation of particles throughout the fluidics system.

Maintains System Cleanliness

Attune® Focusing Fluid is a sterile, buffered, azide-free support reagent that contains a surfactant that helps clean the fluidic lines of the Attune® Acoustic Focusing Cytometer. The Attune® Focusing Fluid was designed to minimize bubble formation and to prevent build-up of cellular material in the flow cell, resulting in less instrument clogging.

Convenient

The ready-to-use 1X focusing fluid was designed to eliminate the need for dilution prior to use, as well as access to a purified water system. The convenient 10 L packaging provides enough focusing fluid to operate for at least 10 days (the average focusing fluid consumption per day is less than 1 L) and has a spigot to to make filling of the onboard fluidics tanks easier.

High Quality

Attune® Focusing Fluid is prepared, filtered, and packaged in a sterile environment to ensure the highest quality. Each batch is traceable by its lot number designation and has been validated for use on the Attune® Acoustic Focusing Cytometer.

Achieve functional knockout in human primary T-cells

T cell receptor knockout using TrueGuide Synthetic gRNAs. Human T cells were isolated and activated using Dynabeads magnetic beads (Cat. Nos. 11344D and 11141D, respectively) and then transfected with TrueCut Cas9 Protein v2 and TrueGuide Synthetic gRNA for T cell receptor alpha (TRAC) or beta (TRBC) regions using the Neon Transfection System. Following transfection, editing efficiency was measured by the GeneArt Cleavage Detection assay (a) or by measuring % T cell receptor negative (TCR–) cells using the Attune NxT Flow Cytometer (b). Cells analyzed by flow cytometry were stained with a T cell receptor–specific antibody conjugated to PE (c).