Goat anti-Mouse IgG Fc Recombinant Secondary Antibody/山羊抗小鼠IgG Fc重组二抗

货号：SA5-10227

规格：100 µg

价格：2732

产品类型：二抗

品牌：Thermo Fisher

抗原：Peptide corresponding to Mouse IgG.

物种：小鼠

宿主：山羊

抗体亚型：IgG

克隆号：RMG06

荧光染料：其它

类型:	二抗	同型对照：
浓度：	1 mg/mL	用法：	0.05-1 µg/mL(ELISA)；0.5-2 µg/mL(ICC)；0.5-2 µg/mL(IF)；0.5-2 µg/mL(IHC)

产品详细信息This antibody reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG.Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include: better specificity, animal origin-free formulation, and more lot-to-lot consistency.靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG Fc Secondary Antibody (SA5-10227) in ELISAELISA of mouse immunoglobulins shows Goat Anti-Mouse IgG Fc Secondary Antibody (Product # SA5-10227) reactsto the Fc region of mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of Goat Anti-Mouse IgG Fc Secondary Antibody (Product # SA5-10227) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody. Mouse IgG Fc Secondary Antibody (SA5-10227) in ELISAA titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of Goat Anti-Mouse IgG Fc Secondary Antibody (Product # SA5-10227) was used as the primary antibody. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody

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