DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin/FLAG标签抗体

货号：MA1-91878-BTIN

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：恒河猴/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG

荧光染料：Biotin

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	Mouse / IgG2b
免疫原性：	Synthetic peptide (DYKDDDDK) coupled to KLH.	用法：	1:200 (ICC);1:200（IF); 1:200 (IHC);1µg/mL（WB)

Product Specific InformationMA1-91878-BTIN has been successfully used for Western blot, IF and IP applications.similar epitope as the FLAG tag from SigmaBackground/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

DYKDDDDK Tag Antibody (MA1-91878-BTIN) in WB1:1000 (1 µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). DYKDDDDK Tag Antibody (MA1-91878-BTIN) in WB Western blot analysis of FLAG Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

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参考文献：

1.PloS one Truncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis. View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer 2.The Journal of biological chemistry Class I Histone Deacetylase HDAC1 and WRN RECQ Helicase Contribute Additively to Protect Replication Forks upon Hydroxyurea-induced Arrest. "MA1-91878-BTIN was used in western blot to propose that WRN interacts with HDACs to facilitate activity of stalled replication forks under conditions of replication stress" AuthorsKehrli K,Phelps M,Lazarchuk P,Chen E,Monnat R,Sidorova JM

技术参数

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