DYKDDDDK Tag Monoclonal Antibody (FG4R), DyLight 680/FLAG标签抗体

货号：MA1-91878-D680

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：IgG

荧光染料：DyLight 680

点击Invitrogen标签抗体集，查看更多标签抗体

抗体类型:	单抗	同型对照：	Mouse / IgG2b
免疫原性：	Synthetic peptide (DYKDDDDK) coupled to KLH.	用法：	1:25-1:100 (ICC);1:25 - 1:100（IF); 1 µg/mL（WB)

Product Specific InformationMA1-91878-D680 has been successfully used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.similar epitope as the Flag tag from Sigma.Background/Target InformationDYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

DYKDDDDK Tag Antibody (MA1-91878-D680) in WB Western blot analysis of FLAG Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey. DYKDDDDK Tag Antibody (MA1-91878-D680) in IFImmunofluorescent analysis of HeLa cells transfected with a construct containing a FLAG Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated FLAG Epitope Tag monoclonal antibody (Product # MA1-91878-D680) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

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参考文献：

1.Genome researchU2AF1 mutations alter splice site recognition in hematological malignancies.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.PloS oneTruncated Bovine Integrin Alpha-v/Beta-6 as a Universal Capture Ligand for FMD Diagnosis.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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