MitoTracker™ Orange CMTMRos - Special Packaging
货号:M7510
规格:20 x 50 µg
价格:5060
产品类型:细胞凋亡检测
品牌:Thermo Fisher
物种:其它
宿主:其它
抗体亚型:其它
荧光染料:其它
MitoTracker® Orange CMTMRos is an orange-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation. |
数据: |
A549 spheroid mounted with Prolong Glass and imaged with EVOS FL Auto 2A549 spheroid grown in Nunclon Sphera microplates (Cat. No. 174925) at 1000 cells/well. Live cells were labeled with NucBlue Live ReadyProbes Reagent (Cat. No. R37605), MitoTracker Orange (Cat. No. M7510), and CellROX Deep Red Reagent for oxidative stress detection (Cat. No. C10422) for 1 hour at 37°C. Cells were then fixed with 4% PFA, transferred to a #1.5 coverslip, and mounted in ProLong Glass. Sample was allowed to cure overnight exposed to air, and the coverslip was mounted to the slide using 100% glycerol. Image acquired with EVOS FL Auto 2 (AMAFD2000) using an Olympus 10X air objective (AMEP4753). |
相关产品: |
细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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