6x-His Tag Monoclonal Antibody (HIS.H8), Biotin
货号：MA1-21315-BTIN
规格：50 µL
价格：2753
产品类型：标签抗体
品牌：Thermo Fisher
物种：恒河猴/小鼠/大鼠
宿主：小鼠
抗体亚型：IgG
荧光染料：Biotin
抗体类型: | 标签抗体 | 同型对照： | IgG2b |
免疫原性： | 6x His synthetic peptide. | 用法： | 1:500-1:2000 (ICC);1:500-1:2000（IF);1:500（WB) |
Product Specific InformationMA1-21315-BTIN has been successfully used for Western blot, ELISA, IF and IPBackground/Target InformationThe 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| 6x-His Tag Antibody (MA1-21315-BTIN) in IFLEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).6x-His Tag Antibody (MA1-21315-BTIN) in WBWestern blot analysis was performed by loading 125 ng (Lane 2), 60 ng (Lane 2), 30 ng (Lane 3) and 15 ng (Lane 4) of 6x-His tagged FoxP1 protein. The blot was probed with Anti-6x-His Tag Mouse Monoclonal Antibody (Product # MA1-21315BTIN, 1:500 dilution). A 18 kDa band corresponding to FoxP1- 6x-His Tag was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. This is followed by incubating the membrane with Poly-HRP Streptavidin (Product # N200, 1:10,000 dilution). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). |
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参考文献： |
| 1.Nucleic acids research Conservative site-specific and single-copy transgenesis in human LINE-1 elements. View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer |
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