GFP Monoclonal Antibody (GF28R), HRP/GFP抗体

货号：MA5-15256-HRP

规格：50 µL

价格：2753

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：HRP

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.	用法：	1:500-1:2000(WB)

Product Specific InformationMA5-15256-HRP has been successfully used in Western blotting applications with GFP tagged samples.Background/Target InformationThe jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organinsms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcelluclar compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used etensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GFP Antibody (MA5-15256-HRP) in WBWestern blot analysis of GFP was performed by loading various amounts of recombinant GFP per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GFP monoclonal antibody (Product # MA5-15256-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

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参考文献：

1.Developmental cellpiRNAs and piRNA-Dependent siRNAs Protect Conserved and Essential C. elegans Genes from Misrouting into the RNAi Pathway."MA5-15256-HRP was used in Western Blotting to uncover a role for piRNAs in protecting essential genes from RNA silencing."AuthorsPhillips CM,Brown KC,Montgomery BE,Ruvkun G,Montgomery TA2.eLifec-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility."MA5-15256-HRP was used in western blot to study the role of the chemotaxis machinery in the partitioning of c-di-GMP concentrations in Pseudomonas aeruginosa"AuthorsKulasekara BR,Kamischke C,Kulasekara HD,Christen M,Wiggins PA,Miller SI3.PLoS geneticsAn inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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