TAMRA Monoclonal Antibody (5G5)/TAMRA 抗体

货号：MA1-041

规格：100 µg

价格：5442

产品类型：标签抗体

品牌：Thermo Fisher

物种：Tag

宿主：小鼠

抗体亚型：其它

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG1
免疫原性：	TAMRA molecule	用法：	10 µg（IP）；1:1000(WB)

Product Specific InformationMA1-041 detects TAMRA in samples and has been used in western blotting and immunoprecipitation applications.Background/Target InformationThe red-fluorescent tetramethylrhodamine (TAMRA) azide can be used as a reactive-azido group (Staudinger reagents) that facilitates multiplex labeling when used with phosphine- or alkyne-derivatized tags. In addition, TAMRA fluorophore has been used to label and detect active serine hydrolases in samples by fluorescent gel imaging, capillary electrophoresis or mass spectrometry.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TAMRA Antibody (MA1-041) in IPImmunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5 µg protein with 10 µg of mouse monoclonal antibody recognizing TAMRA (Product # MA1-041) or Mouse IgG, whole molecule (Product # 31202) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50 µL Protein A/G Plus Agarose (Product # 20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532 nM, Normal Sensitivity. TAMRA Antibody (MA1-041) in WBWestern blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing TAMRA (Product # MA1-041) at a dilution of 1:1000 for 1hour at room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product # 34087).

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参考文献：

1.Nature communicationsVCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation."MA1-041 was used in western blot to show how ATL1 and VCP work together to regulate endoplasmic reticulum and protein synthesis for dendritic spine formation"AuthorsShih YT,Hsueh YP2.Proceedings of the National Academy of Sciences of the United States of AmericaHigh-resolution functional proteomics by active-site peptide profiling."MA1-041 was used in immunoprecipitation to enrich for TAMRA-labeled peptides and then eluted and analyzed LC-MS/MS."AuthorsOkerberg ES,Wu J,Zhang B,Samii B,Blackford K,Winn DT,Shreder KR,Burbaum JJ,Patricelli MP

技术参数

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