Cyclophilin A Polyclonal Antibody/亲环素A内参抗体

货号：PA1-025

规格：400 µL

价格：5442

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

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抗体类型:	多抗	同型对照：	IgG
免疫原性：	Purified recombinant human CyPA.	用法：	1:250（ICC）；1:250（IF）；1:100-1:1000(WB)

Product Specific InformationPA1-025 detects recombinant human cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. PA1-025 also detects the CyPA protein from CHO cells.PA1-025 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a prominent ~18 kDa protein representing recombinant human CyPA.The PA1-025 immunogen is recombinant human CyPA expressed in E. coli.Background/Target InformationImmunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Cyclophilin A Antibody (PA1-025) in WBWestern blot analysis of Cyclophilin A was performed by loading 25 µg of Hela (lane 1), K562 (lane 2), HepG2 (lane 3) and NIH-3T3 (lane 4) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Cyclophilin A polyclonal antibody (Product # PA1-025) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~18 kDa. Cyclophilin A Antibody (PA1-025) in IFImmunofluorescent analysis of Cyclophilin A (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin A monoclonal antibody (Product # PA1-025) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x. Cyclophilin A Antibody (PA1-025) in IFImmunofluorescent analysis of Cyclophilin A (green) showing staining in the in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin A polyclonal antibody (Product # PA1-025) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

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参考文献：

1.RetrovirologyMultiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5α-mediated restriction."PA1-025 was used in western blot to investigate the mechanism for the evasion of Rh TRIM5alpha restriction by simian immunodeficiency viru core protein"AuthorsKono K,Song H,Yokoyama M,Sato H,Shioda T,Nakayama EE2.Cancer epidemiology, biomarkers and prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive OncologyProteomic profiling identifies cyclooxygenase-2-independent global proteomic changes by celecoxib in colorectal cancer cells."PA1-025 was used in western blot to find new celecoxib-mediated markers in the COX-2-deficient colorectal cancer cell line"AuthorsLou J,Fatima N,Xiao Z,Stauffer S,Smythers G,Greenwald P,Ali IU3.The Journal of biological chemistryCell surface expression of CD147/EMMPRIN is regulated by cyclophilin 60."PA1-025 was used in western blot to demonstrate the role of Cyp60 in the CD147 translocation to the cell surface."AuthorsPushkarsky T,Yurchenko V,Vanpouille C,Brichacek B,Vaisman I,Hatakeyama S,Nakayama KI,Sherry B,Bukrinsky MI4.Journal of virologyCyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells."PA1-025 was used in western blot to investigate the role of CypA-CA interaction in regulating HIV-1 replication and infectivity in target human cells."AuthorsHatziioannou T,Perez-Caballero D,Cowan S,Bieniasz PD5.Toxicological sciences : an official journal of the Society of ToxicologyThe characterization and hormonal regulation of kidney androgen-regulated protein (Kap)-luciferase transgenic mice."PA1-025 was used in western blot to study the expression of kidney androgen regulated protein (Kap) in Kap-luc transgenic mice"AuthorsMalstrom SE,Tornavaca O,Meseguer A,Purchio AF,West DB

技术参数

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