CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit
货号：C10427
规格：100 assays
价格：5087
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent® Caspase-3/7 Green Detection Reagent, as well as SYTOX® AADvanced™ Dead Cell Stain.• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7• Easy identification—clearly identify live, dead, and apoptotic cell populations• Quick analysis—washing and fixation is not required• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasersView a selection guide for all apoptosis assays for flow cytometryCellEvent® Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ~511/533 nm. When used together with the SYTOX® AADvanced™ Dead Cell Stain, apoptotic cells can be easiliy discriminated from live and necrotic cells.Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis. For more information, see Apoptosis Reagents in Flow Cytometry. |
| 数据： |
| Caspase activity detection in Jurkat cells using the CellEvent® Caspase-3/7 Green Flow Cytometry Assay Kit on the Attune® Flow Cytometer.Jurkat cells (T-cell leukemia, human) were treated with (A) DMSO or (B) 10 µM camptothecin for 3 hours before labeling with the CellEvent® Caspase 3/7 Green Flow Cytometry kit. Stained samples were analyzed on the Attune® Acoustic Focusing Cytometer equipped with a 488-nm laser, and fluorescence emission was collected using a 530/30 BP filter for CellEvent® Caspase 3/7 Green Detection Reagent and a 690/50BP filter for SYTOX® AADvanced™ stain, respectively. Note that the treated cells have a higher percentage of apoptotic cells (panel B) than the basal level of apoptosis seen in the control cells (panel A). A =apoptotic cells, L = viable cells, N = necrotic cells. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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