Cyclophilin B Polyclonal Antibody/亲环素D内参抗体

货号：PA1-027A

规格：100 µg

价格：4050

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：其它

荧光染料：其它

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抗体类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB.	用法：	3-5 µg/1x10^6 cells（Flow）；1:100-1:1000（ICC）;1:100-1:1000（IF）；1:50-1:500（IHC（P））;1.0-2.0 µg/mL(WB)

Product Specific InformationPA1-027A detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.PA1-027A has been successfully used in Western blot procedures, IF/ICC and IHC (P). By Western blot, this antibody detects a ~19 kDa protein representing CyPB from rat liver extract.The PA1-027A immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1-027A immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.Background/Target InformationImmunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP).Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Cyclophilin B Antibody (PA1-027A) in IFImmunofluorescent analysis of Cyclophilin B (green) showing staining in the in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

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