CellEvent™ Caspase-3/7 Green Detection Reagent
货号：C10423,C10723
规格：100 µL,25 µL
价格：9446,2883
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| CellEvent™ Caspase-3/7 Green Detection Reagent is a novel fluorogenic substrate for activated caspase-3/7 that is compatible with both live cell and fixed imaging, with absorption/emission maxima of ~502/530 nm. Activation of caspase-3 is an essential event during apoptosis, making this an optimized reagent for analysis of apoptotic cells.See more tools for detection of apoptotic cells >• Optimized caspase-3/7 substrate for apoptosis analysis• Easy to use—add and read; no cell lysis required• Use for time course measurements; easily select the time point of interest• Compatible with both live cell fluorescence-imaging and formaldehyde-based fixation methods• Multiplex enabled—combine with other fluorescent reagents to confirm apoptosis in the same cell or cell populationSubstrate specificsCellEvent Caspase-3/7 Green Detection Reagent is a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye with absorption/emission maxima of ~502/530 nm. The DEVD peptide sequence is a cleavage site for caspase-3/7, and the conjugated dye is non-fluorescent until cleaved from the peptide and bound to DNA.Principles of actionCellEvent Caspase-3/7 Green Detection Reagent is intrinsically non-fluorescent as the DEVD peptide inhibits the ability of the dye to bind to DNA. However, after activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved, enabling the dye to bind to DNA and produce a bright, fluorogenic response. The fluorescent emission of the dye when bound to DNA is ~530 nm and can be observed using a standard FITC filter set.Simple three-step protocolTo use CellEvent Caspase 3/7 Detection Reagent, simply add it to cells, incubate 30 minutes, and visualize (see figure). Apoptotic cells with activated caspase-3/7 will have bright green nuclei, while cells without activated caspase 3/7 will have minimal fluorescent signal (see figure).Assay versatility allows for live cell detection and fixationThis robust assay enables the examination of caspase-3/7 activation in live cells. Furthermore, since wash steps are not necessary for detection, fragile apoptotic cells typically lost during wash steps are preserved. Importantly, the fluorescent signal from CellEvent Caspase 3/7 Detection Reagent can survive fixation and permeabilization. This allows for the flexibility to perform end-point assays and probe for other proteins of interest using immunocytochemistry. |
| 数据： |
| Traditional fluorescence imaging of oxidative stress and apoptosisHeLa cells were treated with or without 0.5 µM staurosporine for 2 or 4 hours in the presence of 7.5 µM fluorogenic caspase 3/7 substrate. Cells were then stained with 5 µM Far Red ROS Sensor and Hoechst 33342 for 30 minutes at 37°C, then washed with warm DPBS. Cells were imaged immediately on a Zeiss Axiovert® inverted microscope using a 40x objective. Increased oxidative stress was observed at 2 hours after treatment (magenta) while caspase 3/7 activation was not observed until 4 hours after treatment (green) as shown in representative cells above. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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