CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent
货号：R37111
规格：1 kit
价格：3762
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| CellEvent® Caspase-3/7 Green ReadyProbes® Reagent is a fluorogenic, no-wash indicator of activated caspase-3/7 for live- and fixed-cell applications. Activation of caspase-3 is an early indicator of apoptosis and CellEvent® Caspase-3/7 Green reagent allows rapid and sensitive detection of cells destined for cell death.• Simple and fast no-wash protocol helps preserve delicate apoptotic cells• Compatible with both live-cell fluorescence imaging and formaldehyde-based fixation methods• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette• Stability at room temperature—keep handy at your scope or cell culture area.See other ReadyProbes® reagents for cell stainingLearn more about other assays for apoptosisCell imaging applications CellEvent® Caspase-3/7 Green reagent is a four amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that is non-fluorescent when not bound to DNA. The CellEvent® Caspase-3/7 Green reagent is intrinsically non-fluorescent, as the DEVD peptide inhibits binding of the dye to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind DNA, generating a bright green fluorescence. The fluorescence emission of the dye when bound to DNA is 530 nm and can be observed using a standard FITC filter set.Suggestions for use• In most cases, 2 drops/ml and an incubation time of 30 minutes is sufficient for bright nuclear staining of apoptotic cells; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.• If desired, combine CellEvent™ Caspase-3/7 Green ReadyProbes® reagent with a red or far-red nuclear stain for a total cell count• CellEvent™ Caspase-3/7 Green dye is excited with a maximum at 502 nm and has an emission maximum at 530 nm. It is detected through standard GFP and FITC filters. |
| 数据： |
| Capturing apoptosis with CellEvent® Green and the EVOS® FL Auto imaging systemU-2 OS cells were treated with 250 nM staurosporine for 4 h and apoptotic cells were detected with CellEvent® Caspase-3/7 ReadyProbes reagent according to the protocol. Using the automated scanning function on the EVOS® FL Auto imaging system, 55 images covering an entire 96-well plate were acquired at 10x magnification (AMEP4623). A whole-well montage was then generated using the tile/stitch function. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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