Image-iT™ LIVE Red Poly Caspases Detection Kit, for microscopy
货号：I35101
规格：1 kit
价格：6013
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：其它
| The Image-iT™ LIVE Red Poly Caspases Detection Kit employs a novel approach to detect active caspases that is based on a fluorescent inhibitor of caspases (FLICA™) methodology, essentially an affinity label. The reagent associates a fluoromethyl ketone (FMK) moiety, which can react covalently with a cysteine, with a caspase-specific amine acid sequence. For poly caspases, this recognition sequence is valine-alanine-aspartic acid (VAD). A sulforhodamine group (SR) is attached as a reporter. The FLICA reagent is thought to interact with the enzymatic reactive center of an activated caspase via the recognition sequence, and then to attach covalently through the FMK moiety. The FLICA inhibitor is cell permeant and noncytotoxic. Unbound FLICA molecules diffuse out of the cell and are washed away; the remaining red-fluorescent signal is a direct measure of the amount of active caspase that was present at the time the inhibitor was added. |
| 数据： |
| Live HeLa cells visualized using the Image-iT® LIVE Red Poly Caspase Detection Kit.Live HeLa cells visualized using the Image-iT® LIVE Red Poly Caspase Detection Kit (I35101). Cells were treated with staurosporine to induce apoptosis, and FLICA reagent (SR-VAD-FMK, left) was added. Cells were then loaded with SYTOX® Green dye (middle) and Hoechst 33342 (blue fluorescence, right) and analyzed by fluorescence microscopy. Apoptotic, non-apoptotic, and dead cells are all visible in the same field. |
| 相关产品： |
| 细胞周期检测细胞凋亡检测细胞增殖活性检测 |
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