beta Tubulin Loading Control Monoclonal Antibody (BT7R), Alexa Fluor 555

货号：MA5-16308-A555

规格：50 µl

价格：4050

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 555

类型:	单抗	同型对照：	IgG2a
免疫原性：	KLH conjugated peptide of the N-terminus of Beta-Tubulin.	用法：	1:25-1:100(ICC);1:25-1:100(IF);1:500(WB)

Background/Target InformationBeta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

beta Tubulin Loading Control Antibody (MA5-16308-A555) in IFImmunofluorescent analysis of beta tubulin (orange) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with AlexaFluor 555 conjugated beta-tubulin monoclonal antibody (Product # MA5-16308-A555) at a dilution of 1:50 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. beta Tubulin Loading Control Antibody (MA5-16308-A555) in IFImmunofluorescence analysis of beta tubulin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Tubulin Loading Control Monoclonal Antibody (BT7R), Alexa Fluor 555 (Product # MA5-16308-A555) at 1:25 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: green) was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the isotype control. The images were captured at 60X magnification. beta Tubulin Loading Control Antibody (MA5-16308-A555) in WBWestern blot analysis of beta-Tubulin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor555-conjugated beta-Tubulin monoclonal antibody (Product # MA5-16308-A555) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system.

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