Clathrin Heavy Chain Monoclonal Antibody (X22)

货号：MA1-065

规格：100 µL

价格：5670

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：其它

荧光染料：其它

类型:	单抗	同型对照：	IgG1
免疫原性：	Purified human brain clathrin heavy chain.	用法：	1/200（Flow）;1:100-1:500(WB);1:1000(IF);1:100(IHC)

Product Specific InformationMA1-065 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells.MA1-065 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody recognizes a single ~180 kDa protein representing the clathrin heavy chain from bovine brain extract. Immunoprecipitation of triskelions with MA1-065 allows both the clathrin heavy chain and the associated light chain polypeptides to be examined.The MA1-065 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1-065 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.Target InformationClathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Clathrin Heavy Chain Antibody (MA1-065) in IHCImmunohistochemistry was performed on normal biopsies of deparaffinized Human colon tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Clathrin, Heavy chain (Product # MA1-065) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Clathrin Heavy Chain Antibody (MA1-065) in IFImmunofluorescent analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) (Product # MA1-065) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain (Product # MA1-065) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

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参考文献：

1.Frontiers in molecular neuroscienceAn Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.The Journal of biological chemistrySurface Toll-like receptor 3 expression in metastatic intestinal epithelial cells induces inflammatory cytokine production and promotes invasiveness.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.PloS oneEndocytic Pathways Used by Andes Virus to Enter Primary Human Lung Endothelial Cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4.Nature communicationsInfluenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5.VirologyCaveolin- and clathrin-independent entry of BKPyV into primary human proximal tubule epithelial cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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