CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), Functional Grade, eBioscience™/CD49d (Integrin alpha 4) 单克隆 (9F10), 功能抗体, eBioscience™
货号：16-0499-81,16-0499-85,16-0499-38,16-0499-025,16-0499-050
规格：50 µg,500 µg,5 mg,25 mg,50 mg
价格：1196,3496,24089,72266,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD49d
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：9F10
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 1 µg/test（Flow）;Assay-Dependent（功能检测） |
产品详细信息Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis. 9F10 has also been reported in in vitro functional assays.Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.仅用于科研。不用于诊断过程。
数据 |
| CD49d (Integrin alpha 4) Antibody (16-0499-81) in FlowStaining of normal human peripheral blood cells with Anti-Human CD49d (Integrin alpha 4) PE.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis. |
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参考文献： |
| 流式1.VirologyImpact of antibody quality and anamnestic response on viremia control post-challenge in a combined Tat/Env vaccine regimen in rhesus macaques."16-0499 was used in Flow cytometry/Cell sorting to evaluate the factors regulating the immunogenicity and protective efficacy against viruses in a Tat/Env vaccine regimen."AuthorsDemberg T,Brocca-Cofano E,Kuate S,Aladi S,Vargas-Inchaustegui DA,Venzon D,Kalisz I,Kalyanaraman VS,Lee EM,Pal R,DiPasquale J,Ruprecht RM,Montefiori DC,Srivastava I,Barnett SW,Robert-Guroff M |
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