PMP70 Polyclonal Antibody, Alexa Fluor 647
货号：PA1-650-A647
规格：50 µl
价格：4223
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 647
类型: | 多抗 | 同型对照： | IgG |
免疫原性： | Synthetic Peptide: C N(644) Y E F K K I T E D T V E F G S(659) | 用法： | 1-10 µg/mL(IF);1-10µg/mL(ICC) |
Product Specific InformationPA1650A647 immunizing peptide corresponds to amino acid residues 644-659 from rat PMP70. This sequence is completely conserved in mouse PMP70 and differs from human PMP70 by a single amino acid substitution. The immunizing peptide (Cat. # PEP-038) is available for use in neutralization and control experiments.Target InformationPeroxisomes are single membrane organelles which are involved with many important biochemical pathways and are found in almost all eukaryotic cells. Various toxins including, phenols, alcohols, and aldehydes are targeted for modification by peroxisomes. Long chain fatty acid metabolism via beta-oxidation is also mediated by this organelle. Loss of peroxisomal functions may result in diseases such as Zellweger syndrome, hyperpipecolic acidemia, neonatal adrenoleukodystrophy, and infantile Refsum""e;s disease. Peroxisomal membrane protein 70 (PMP70) is an abundant, integral membrane protein of the peroxisome. This protein is induced by treatment with hypolipidemic agents in parallel with peroxisome proliferation and stimulation of the peroxisomal beta-oxidation enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
PMP70 Antibody (PA1-650-A647) in IFImmunofluorescent analysis of PMP-70 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a PMP-70 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-650-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
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