ATP Synthase beta Monoclonal Antibody (4.3E8.D10)
货号：MA1-930
规格：100 µg
价格：5468
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：小鼠
抗体亚型：其它
荧光染料：其它
类型: | 多抗 | 同型对照： | IgG1 |
免疫原性： | Intact rat mitochondria. | 用法： | 1:100-1:1000(IF);1:100-1:1000(ICC);2-5 µg(IP);1-2 µg/mL(WB) |
Product Specific InformationMA1-930 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.MA1-930 has been successfully used in immunofluorescence, western blot, and immunoprecipitation procedures. By immunoprecipitation, this antibody detects an 50 kDa protein representing ATP synthase from solubilized rat brain mitochondria.Target InformationATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer.The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
ATP Synthase beta Antibody (MA1-930) in IFImmunofluorescent analysis of ATP Synthase beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a ATP Synthase beta monoclonal antibody (Product # MA1-930) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). ATP Synthase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.ATP Synthase beta Antibody (MA1-930) in IFImmunofluorescent analysis of ATP Synthase beta (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ATP Synthase beta mouse monoclonal antibody (Product # MA1-930), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. |
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参考文献： |
| 1.The Journal of clinical investigationPINK1 deficiency impairs mitochondrial homeostasis and promotes lung fibrosis.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.FEMS microbiology lettersHuman brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH."MA1-930 was used in western blot to investigate the molecular mechanism for the binding of E.coli to human brain microvascular endothelial cells"AuthorsShin S,Kim KS3.The American journal of pathologyThe Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach."MA1-930 was used in western blot to study the progression of endothelial cell dysfunction."AuthorsAddabbo F,Ratliff B,Park HC,Kuo MC,Ungvari Z,Csiszar A,Ciszar A,Krasnikov B,Krasnikof B,Sodhi K,Zhang F,Nasjletti A,Goligorsky MS4.The Journal of biological chemistryPolyamine transport by mammalian cells and mitochondria: role of antizyme and glycosaminoglycans."MA1-930 was used in western blot to investigate the roles of antizyme and glycosaminoglycans during the polyamine transport in mammalian cells and mitochondria."AuthorsHoshino K,Momiyama E,Yoshida K,Nishimura K,Sakai S,Toida T,Kashiwagi K,Igarashi K5.American journal of physiology. Gastrointestinal and liver physiologyExpression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress."MA1-930 was used in immunocytochemistry to study the association between the upregulation of BNIP3 and the hepatocyte cell death"AuthorsMetukuri MR,Beer-Stolz D,Namas RA,Dhupar R,Torres A,Loughran PA,Jefferson BS,Tsung A,Billiar TR,Vodovotz Y,Zamora R |
QQ:1749072012