ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor 488

货号：MA1-930-A488

规格：50 µl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 488

类型:	多抗	同型对照：	IgG
免疫原性：	Intact rat mitochondria	用法：	1-10 µg/mL(IF);1-10µg/mL(ICC)

Product Specific InformationMA1930A488 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.Target InformationATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer.The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

ATP Synthase beta Antibody (MA1-930-A488) in IFImmunofluorescent analysis of ATP Synthase Beta (green) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ATP Synthase beta mouse monoclonal antibody, AlexaFluor 488 conjugate (Product # MA1-930-A488), at a concentration of 5 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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