Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor 488

货号：MA3-38200-A488

规格：50 µl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：其它

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	IgG1, kappa
免疫原性：	Rat Cytochrome C.	用法：	1-10 µg/mL(IF);1-10µg/mL(ICC)

Product Specific InformationThis antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.Target InformationCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Cytochrome C Antibody (MA3-38200-A488) in IFImmunofluorescent analysis of Cytochrome C (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cytochrome C Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA7-00184-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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