KDEL Polyclonal Antibody, Alexa Fluor 488

货号：PA1-013-A488

规格：50 µl

价格：4426

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 488

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94	用法：	1-10 µg/mL(IF);1-10 µg/mL(ICC);2 µg/mL(WB)

Product Specific InformationPA1013A488 detects KDEL from human, rat, mouse and hamster.The PA1013A488 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.Target InformationThe sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

KDEL Antibody (PA1-013-A488) in IFImmunofluorescent analysis of KDEL/ER (green) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a KDEL/ER Rabbit polyclonal antibody, AlexaFluor 488 conjugate (Product # PA1-013-A488), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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参考文献：

1.The Journal of biological chemistryHyaluronan Rafts on Airway Epithelial Cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.The Journal of biological chemistryPrimary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.The Journal of biological chemistryDifferentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.View published figure on By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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