产品介绍 |
| Goat anti-Histidine tag antibodyrecognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Goat anti-Histidine tag antibody may not detect all N-Terminal (NT) histidine tagged proteins. Goat anti-Histidine tag antibody has been shown to be suitable for use on cytospins. |
产品详情 |
Target Species | Synthetic Peptide |
Product Form | Purified IgG - liquid |
AntiserumPreparation | Antiserum to Histidine Tag was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Immunogen | Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H |
Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Acknowledgements | His-tag is a registered trademark of EMD Biosciences. |
存储条件 |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
应用 |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA1 | √ | 1/1000 | 1/30000 |
Western Blotting | √ | 0.5 | 1.0ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1We recommend a dilution range of 1/1000 to 1/30000 for use in indirect ELISA. When coating the antibody directly on to a plate, Bio-Rad recommend a dilution range to 1/100 to 1/500.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
可用二抗 |
Description | Product Code | Applications | Pack Size |
Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
QQ:1749072012