Cyclophilin B Polyclonal Antibody, Alexa Fluor 488
货号：PA1-027A-A488
规格：50 µl
价格：4050
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 488
类型: | 多抗 | 同型对照： | IgG |
免疫原性： | Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB | 用法： | 1-10 µg/mL(IF);1-10 µg/mL(ICC) |
Product Specific InformationPA1027AA488 detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.The PA1027AA488 immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1027AA488 immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.Target InformationImmunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP).Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| Cyclophilin B Antibody (PA1-027A-A488) in IFImmunofluorescent analysis of Cyclophilin B (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Cyclophilin B Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-027A-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. |
推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-17735.htm
QQ:1749072012