Histone H3 Antibody, Alexa Fluor 488 (17H2L9), ABfinity™ Rabbit Monoclonal View (167) other Histone H3 antibodies

货号：MA7-02023-A488

规格：50 µl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	IgG
免疫原性：	Synthetic peptide corresponding to the N-terminal region of human Histone H3 (aa 4-11)	用法：	5 µg/mL(IF);5 µg/mL(ICC)

Product Specific InformationABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.Since it is highly conserved across species, the antibody may react with many other species.Target InformationHistone octamers are an essential component of the nucleosomal complex with key roles in chromatin packaging and target gene transcription. They undergo various post-translational modifications including methylation, acetylation and phosphorylation to facilitate chromatin regulation. Modified histones display varied distribution patterns at the heterochromatin and the euchromatin, hence defining actively transcribed or repressed genes in the eurochromatin and the 'silenced' loci in the heterochromatin. These modifications in turn serve as epigenetic markers for transcriptional status of a gene and landing sites for transcriptional complexes, fundamental to understanding several biological processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Histone H3 Antibody (MA7-02023-A488) in IFImmunofluorescent analysis of Histone H3 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Histone H3 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA7-02023-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification. Histone H3 Antibody (MA7-02023-A488) in IFImmunofluorescence analysis of Histone H3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H3 Antibody, Alexa Fluor 488 (17H2L9), ABfinity™ Rabbit Monoclonal (Product # MA7-02023-A488) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the isotype control. The images were captured at 60X magnification.

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