PSMB6 Polyclonal Antibody, Alexa Fluor 555

货号：PA1-978-A555

规格：50 µl

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：Alexa Fluor 555

类型:	多抗	同型对照：	IgG
免疫原性：	Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)	用法：	1-10 µg/mL(ICC);1-10 µg/mL(IF)

Product Specific InformationPA1978A555 detects proteasome 20S Y from purified bovine and human 26S proteasome samples.The PA1978A555 immunizing peptides correspond to amino acid residues 41-58 from human proteasome 20S Y and amino acid residues 74-91 from frog. These sequences are completely conserved in mouse, rat, and zebrafish. PA1978A555 immunizing peptide (Cat. # PEP-173) is available for use in neutralization and control procedures.Target InformationProteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation.Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelman""e;s syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

PSMB6 Antibody (PA1-978-A555) in IFImmunofluorescent analysis of Proteasome 20S (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Proteasome 20S Polyclonal Antibody, AlexaFluor 555 conjugate (Product # PA1-978-A555) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

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