EZ-Link Micro Sulfo-NHS-Biotinylation Kit/EZ-Link微型磺基-NHS-生物素化试剂盒
货号：21925
规格：8 reactions
价格：6932
产品类型：蛋白标记
品牌：Thermo Fisher
| The Thermo Scientific EZ-Link Micro Sulfo-NHS-Biotinylation Kit contains reagents sufficient for 8 biotinylation reactions (e.g., 0.05–0.2 mg antibody per reaction). The EZ-Link Sulfo-NHS-Biotin reagent included in the kit is a short-chain, water-soluble biotinylation reagent used for labeling antibodies, proteins and other molecules that have primary amines.Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is offered in several package sizes and as complete protein labeling kits.We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.N-Hydroxysulfosuccinimide (NHS) estersof biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.Varieties of biotinNHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein. |
| 特点 |
| •Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes•Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes•Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides•Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds•Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved•Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only. |
| 数据 |
Properties of Sulfo-NHS-Biotin Labeling ReagentsThese compounds have simple alkyl spacer arms and water-soluble Sulfo-NHS groups. Reagents with non-sulfonated NHS-ester groups (membrane-permeable) and/or pegylated or cleavable spacer arms are also available. |
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