CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor 488

货号：53-0452-80,53-0452-82

规格：25μg,100μg

价格：1065,2308

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：Alexa Fluor 488

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488, eBioscience™
免疫原性：		用法：	1 μg/test(Flow)；5 μg/mL(IHC (F))；5 μg/mL(IHC (P))；

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This RA3-6B2 antibody has also been tested by immunohistochemistry of frozen tissue sections and can be used at less than or equal to 20 µg/mL. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue sections using low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.

数据

CD45R (B220) Antibody (53-0452-82) in FlowStaining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Human/Mouse CD45R (B220) Alexa Fluor® 488 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in immunologyProapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.2.Frontiers in immunologyATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.3.Nature medicineAdrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.4.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchInactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.5.Nature communicationsmiR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.

技术参数

产品应用 ICC;IF;IHC;Flow

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