FIX & PERM™ Cell Permeabilization Kit

货号：GAS003,GAS004

规格：50 tests,200 tests

价格：933,3348

产品类型：细胞增殖活性检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

描述FIX & PERM® 试剂用于悬浮细胞的固定（试剂 A）和渗透（试剂 B）。 该步骤有利于抗体接近胞内结构并能完整保持细胞的形态学分散特征。 特异性配方可降低背景染色并允许同时添加渗透培养基和荧光染料标记的抗体。分类： GPR声明： 通用实验室试剂。 供实验室使用。

数据

Detection of mouse IFN-gamma and TNF-a cytokine-producing CD4 T cells using the Attune® Acoustic Focusing Cytometer.C57BL/6 splenocytes were left unstimulated or stimulated for 5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. Cells were then surface-stained with anti-mouse CD4 FITC (Cat. No. MCD0401) and subsequently fixed and permeabilized using the FIX & PERM® Cell Permeabilization Kit (Cat. nos. GAS003 or GAS004) and stained intracellularly with anti-mouse interferon gamma (IFN-gamma) PE-Cy®7 conjugate (Cat. no. A18713) and anti-mouse tumor necrosis factor alpha (TNF-a) Pacific Blue™ conjugate (Cat. no. RM90128). Samples were collected using the Attune® Acoustic Focusing Cytometer (blue/violet) with 488 nm excitation and 530/30 nm bandpass emission filter to detect FITC, and 640 nm longpass filter to detect PE-Cy®7 conjugate. 405 nm excitation and 450/40 nm bandpass emission filter were used to detect the Pacific Blue™ fluorescence. PANEL A: IFN-gamma and TNF-a co-staining of total mouse splenocytes that were left unstimulated (left panel) or stimulated (right panel) with PMA and ionomycin in the presence of brefeldin A. PANEL B: CD4 T cell expression of TNF-a (left panel) and IFN-gamma (right panel) after stimulation with the above stated conditions.

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技术参数

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