TRA-1-81 Monoclonal Antibody (TRA-1-81), DyLight 488

货号：MA1-024-D488

规格：100 µL

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

抗原：TRA-1-81

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-81

荧光染料：其它

类型:	单抗	同型对照：	IgM
免疫原性：	human embryonal carcinoma cell line 2102Ep	用法：	1:50-1:500(ICC);1:50-1:500(IF);1:50-1:500(Flow)

Product Specific InformationMA1-024-D488 has been successfully used in ICC/IF and flow cytometry applications on human samples.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-81 Antibody (MA1-024-D488) in IFImunofluorescent analysis of TRA-1-81 (green) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with DyLight 488-conjugated TRA-1-81 monoclonal antibody (left panels, Product # MA1-024-D488) or DyLight 488-conjugated Mouse IgM isotype control antibody (top right panel, Product # MA1-194-D488), at a dilution of 1:250 and incubated for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on an EVOS FL Imaging system at 20X magnification. TRA-1-81 Antibody (MA1-024-D488) in FlowFlow cytometry analysis of TRA-1-81 in HEL 11.4 cells (blue histogram) compared to negative control unstained cells (red histogram). Cells were incubated with a DyLight 488-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-D488) in 5% BSA-PBS at a dilution of 1:100 for 60 minutes for 1 h on ice, washed, and re-suspended into 500 µL of 5% BSA-PBS-buffer containing 10 µL 4% paraformaldehyde prior to FACS analysis. TRA-1-81 Antibody (MA1-024-D488) in IFImmunofluorescent analysis of TRA-1-81 (green) showing membrane staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-D488) in 3% BSA-PBS at a dilution of 1:100 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI. Images were taken at 60X magnification.

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