TRA-1-81 Monoclonal Antibody (TRA-1-81), HRP

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TRA-1-81 Monoclonal Antibody (TRA-1-81), HRP

货号: MA1-024-HRP

规格:100 µL

价格:4070

产品类型:免疫组化一抗

品牌:Thermo Fisher

抗原:TRA-1-81

物种:人

宿主:小鼠

抗体亚型:IgM

克隆号:TRA-1-81

荧光染料:DyLight 650

类型:

单抗

同型对照:

IgM

免疫原性:

human embryonal carcinoma cell line 2102Ep

用法:

1:250-1:1000L(WB)

Product Specific InformationMA1-024-HRP has been successfully used in Western blot applications on human samples.Target InformationTRA-1-81 is a cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are down-regulated during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

TRA-1-81 Antibody (MA1-024-HRP) in WBWestern blot analysis of TRA-1-81 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated TRA-1-81 monoclonal antibody (Product # MA1-024-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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